Cell
Part:BBa_J23017:Design
Designed by: Will Bosworth Group: iGEM2006_Berkeley (2006-07-24)
RLambda traG knockout (graveyarded)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
JL8 Forward oligo to knockout traG-r: atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaagtgCaggctggagctgcttcgaag JL9 Reverse oligo to knockout traG-r: tcatatcgtgatcccctccccttcctcgacggtggccgtctggattgggaaGtagccatggtccatatg
Source
PCR JL8/JL9 on pKD3 (~1000bp, dPN1, Neb2) Electroporate into pKD46xRlambda