Cell

Part:BBa_J23017:Design

Designed by: Will Bosworth   Group: iGEM2006_Berkeley   (2006-07-24)


RLambda traG knockout (graveyarded)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

JL8 Forward oligo to knockout traG-r: atgaagaaccgaaacaacgccgtggggccacagatacgggcgaaagtgCaggctggagctgcttcgaag JL9 Reverse oligo to knockout traG-r: tcatatcgtgatcccctccccttcctcgacggtggccgtctggattgggaaGtagccatggtccatatg


Source

PCR JL8/JL9 on pKD3 (~1000bp, dPN1, Neb2) Electroporate into pKD46xRlambda

References